Nikon Eclipse Ti2-E/Yokogawa CSU-W1 Spinning Disk

 
 
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Inverted confocal spinning-disk microscope dedicated to live cell imaging and applications that require high-speed multidimensional imaging.

 

Specifications

Widefield Illumination

TI2-D-LHLED High-power LED
Lumencor SPECTRA III light engine

Laser Lines

405/488/561/638nm (120/100/100/100mW)

Objectives

CFI Plan Apochromat Lambda 10X/ 0.45/ 4.00
CFI Plan Apochromat Lambda 20X/ 0.75/ 1.00
CFI Plan Apochromat Lambda 40X/ 0.95/ 0.25-0.16
CFI Plan Apochromat Lambda Oil 60X/ 1.40/ 0.13

CFI Plan Apochromat 10XC Glyc/ 0.50/ 2.00
CFI Plan Apochromat Lambda S 25XC Sil/ 1.05/ 0.55
CFI Plan Apochromat Lambda S 40XC Sil/ 1.25/ 0.30

CFI Apochromat LWD Lambda S 20XC WI/ 0.95/ 0.95

Cameras

Photometrics Prime BSI (two cameras)
Nikon DS-Qi2

Filters

Nikon DS-Qi2:
LED-DA/FI/TR/Cy5/Cy7-5X-A Filter Cube 32
LED-CFP/YFP/mCherry-3X-A Filter Cube 32

Photometrics Prime BSI:
447/60 nm BrightLine single-band bandpass (DAPI)
525/50 nm BrightLine single-band bandpass (FITC)
600/52 nm BrightLine single-band bandpass (TRITC)
708/75 nm BrightLine single-band bandpass (Cy5)

Spinning disk unit

Yokogawa CSU-W1
Double disk (25 µm, 50 µm pinhole)
Motorized widefield bypass

Piezo z-drive

Mad City Labs Nanopositioner
Range of motion: 500 µm
Resolution: 1.0 nm

Live cell imaging

Okolab Cage Incubator
TI2-N-WID Water Immersion Dispenser

Software

NIS-Elements

 

Further information: BMBWF Forschungsinfrastruktur 2