Papers CD-MOFA

PUBLICATIONS (PEER REVIEW)

Rossmanith, P. and Wagner, M., 2011. A novel Poisson distribution based approach for testing boundaries of real time PCR assays for food pathogen quantification. In press. J.Food.Prot.

Rossmanith, P., Mester, P., Fruehwirth, K., Fuchs, S., Wagner, M. and 2011. A proof of concept for a novel generation of cellular controls in quantitative molecular pathogen detection. Appl. Environ. Microbiol. DOI:10.1128/AEM.02601-10.

Fruehwirth, K., Fuchs, S., Mester, P., Wagner, M. and Rossmanith, P. 2011. Cloning and characterization of a Δ-prfA Listeria monocytogenes strain containing an artificial single copy genomic internal amplification control (IAC). Food Analytical Methods. DOI: 10.1007/s12161-011-9212-6.

Rossmanith, P. and Wagner, M., 2011. Review. Aspects of systems theory in the analysis and validation of innovative molecular-biological based food pathogen detection methods. Trends in Food Sci. and Technol. 22(2):61-71.

Rossmanith, P. and Wagner, M., 2011. Review. The challenge to quantify Listeria monocytogenes – a model leading to new aspects in molecular biological food pathogen detection. Journal of Applied Microbiology. 110(3):605-17.

Rossmanith, P., Roeder, B., Fruehwirth, K.., Vogl, C., Wagner, M.  2011. Mechanisms of degradation of DNA standards for calibration function in nucleic acid based pathogen detection. Appl. Microbiol. Biot. 2010 Oct 22. 89:407–417.

Rossmanith, P., Hedman, J., Radström, P.; Hoorfar, J., Wagner, M. 2010. Pre-analytical sample preparation and analyte extraction. In: Rapid Detection, Characterization and Enumeration of Food-borne Pathogens. Hoorfar, J. (ed.). ASM Press, N.W. Washington, D.C, USA. In press.

Rossmanith P. and Wagner, M., 2010. Sample preparation for the detection of foodborne pathogens by molecular biological methods. Brul S. and McMeekin T.A. [Eds.]: Tracing pathogens in the food chain. ISBN-13: 978-1-84569-496-8. Woodhead Publishing Limited, Abington Hall, Abington, Cambridge, UK. p. 237-262.

Roeder, B., Fruehwirth, K.., Vogl, C., Wagner, M, Rossmanith, P. 2010. Impact of long-term storage on the stability of standard DNA for nucleic acid based methods. J.Clin.Microbiol. 48(11):4260-4262.

Rossmanith, P., Fuchs, S., and Wagner, M., 2010. The fluorescence characteristics of enrichment media in the wavelength range of real-time PCR thermo-cycler optical path assignments. Food.Anal.Meth. 3, (3): 219-224.

Rossmanith, P., Mester, P., Wagner, M. and Schoder, D., 2010. Demonstration of the effective performance of a combined enrichment/real-time PCR method targeting the prfA gene of Listeria monocytogenes by testing fresh naturally contaminated acid curd cheese. Lett.App.Mircobiol. 2010 Oct;51(4):480-4

Patrick Mester, Martin Wagner, and Peter Rossmanith., 2010. Biased spectroscopic protein quantification in the presence of ionic liquids. In press. Anal. Bioanal. Chem.

Roeder, B., Wagner, M., Rossmanith, P., 2010. Autonomous growth of isolated single L. monocytogenes and S. Typhimurium cells in the absence of growth factors and intercellular contact. Appl. Environ. Microbiol. 2010 Apr;76(8):2600-6.

Patrick Mester, Martin Wagner, and Peter Rossmanith., 2010. Use of ionic liquid-based extraction for recovery of Salmonella Typhimurium and Listeria monocytogenes from food matrices J.Food.Prot. Apr, 73(4):680-7.

Rossmanith, P., Süß, B., Wagner, M., Hein, I., 2007. Development of matrix lysis for concentration of gram positive bacteria from food and blood. J. Microbiol. Methods 69, 504-511.

Mayrl, E., Roeder, B., Mester, P., Wagner M., Rossmanith P., 2009. Broad range evaluation of the matrix solubilization (Matrix Lysis) strategy for direct enumeration of food-borne pathogens by nucleic acids technologies. J.Food.Prot. 2009 Jun;72(6):1225-33.

Rossmanith, P., Frühwirth, K., Süß, B., Schopf, E., Wagner, M., 2009. The use of chromogenic bacteria as coloured substitutes for pathogens: A simple strategy during design and development of a new method for sample pre-treatment. Lett.App.Mircobiol., 2010 Feb;50(2):230-3.

Rossmanith, P., Fuchs, S., and Wagner, M., 2009. The fluorescence characteristics of enrichment media in the wavelength range of real-time PCR thermo-cycler optical path assignments. Food.Anal.Meth. 2009 Oct 22. [Epub ahead of print]

CONGRESS CONTRIBUTIONS (PRESENTATION)

Rossmanith, P., Wagner, M. Quality Standards in Quantitative PCR; Specification, VAlidation, Controls and Standards.     IAFP 2011, 7th European Symposium on Food Safety, Ede, Netherlands, 18-20, May 2011. p 6.

Röder, B., Rossmanith, P., Hein, I., Wagner, M. 
Single bacterial cell manipulation-new challenges in quantitative food microbiology. Annual Meeting ECVPH, Helsinki, 20.-21.09.2007

Rossmanith, P., Süß, B., Wagner, M., Hein, I.
Single DNA molecule detection in real time PCR – a powerful tool in validation of real-time PCR systems and for the generation of absolute DNA standards.
10. Fachsymposium Lebensmittelmikrobiologie, Deutsche Gesellschaft für Hygiene und Mikrobiologie, Stuttgart, 09.-11.04.2008, S. 16.

Matrix lysis: further development of a pre-detection procedure useful both for conventional and molecular analysis of foodborne pathogens
10. Fachsymposium Lebensmittelmikrobiologie, Deutsche Gesellschaft für Hygiene und Mikrobiologie, Stuttgart, 09.-11.04.2008, S. 17.

Röder, B., Wagner, M., Rossmanith, P.
Single bacterial cell manipulation–premises, technical equipment and applications
10. Fachsymposium Lebensmittelmikrobiologie, Deutsche Gesellschaft für Hygiene und Mikrobiologie, Stuttgart, 09.-11.04.2008, S. 45.

Röder, B., Wagner, M., Rossmanith, P.
Mikromanipulation von bakteriellen Zellen und deren Anwendung in    Lebensmittelmatrixmodellen.
31. Jahrestagung der Österr. Gesellschaft für Hygiene, Mikrobiologie und Präventivmedizin, Bad Ischl, 26.-29.05.2008, S. 28-29.

Rossmanith, P., Mayrl E., Röder, B., Wagner, M.
Matrix Lysis - Eine neue Probenvorbereitungsmethode im Zusammenhang eines     alternativen Ansatzes zur Detektion von lebensmittelpathogenen Keimen mittels real -     time PCR, Weiterentwicklung und Ausblick.
31. Jahrestagung der Österr. Gesellschaft für Hygiene, Mikrobiologie und     Präventivmedizin, Bad Ischl, 26.-29.05.2008, S. 29.

Rossmanith, P., Wagner, M.
Statistical data analysis of real-time PCR results derived from single copy amplification. IAFP 2009, 96th Annual Meeting Grapvine,Texas, 12.7.-15.7.2009, p 34.

Mester, P., Röder, B., Mayrl, E., Huehn, S., Wagner, M., Rossmanith, P.
Application of Ionic Liquids for Separation and Concentration of Foodborne Pathogenes from Food for Subsequent Molecular or Cultural Quantification Methods. IAFP 2009, 96th Annual Meeting Grapvine,Texas, 12.7.-15.7.2009, p 38.

Röder, B., Wagner, M., Rossmanith, P.
Single bacterial cell transfer for controlled manipulation of Listeria and Salmonella 50th Conference of Food Hygiene, Garmisch-Partenkirchen, 29.9.-2.10 2009, p 51.

Rossmanith, P., Wagner, M.
Aspects of Systems Theory in the Analysis of Molecular-biological Based Detection Methods. IAFP 2009, 97th Annual Meeting Anaheim, California, 1.8.-4.8.2010, JFPRDR 73 (Sup) p 27.

CONGRESS CONTRIBUTIONS (POSTER)

Glaser, K; Fuchs, S; Wagner, M., and Rossmanith, P.
Chromogenic Indicators for Pathogens Contaminating Food Samples. IAFP 2011, 7th     European Symposium on Food Safety, Ede, Netherlands, 18-20, May 2011. p 11.

Fuchs, S; Mester, P; Wagner, M.; Rossmanith, P.
Ionic Liquids for DNA Quantification out of Gram positive and Gram Negative Bacteria.    IAFP 2011, 7th European Symposium on Food Safety, Ede, Netherlands, 18-    20,     May 2011. p 11.

Fister, S; Fuchs, S; Mester, P; Wagner, M.; Rossmanith, P.
The impact of four buffer systems derived from a matrix solubilisation based sample     preparation protocol for bacteria on the integrity of virus particles.    IAFP 2011, 7th     European Symposium on Food Safety, Ede, Netherlands, 18-    20,     May 2011. p     12.

Schoder, D; Wagner, M.; Rossmanith, P.
Survival and Growth of L. monocytogenes in Quargel (Acid Curd Cheese) Lots Recalled     during the Multinational Listeriosis Outbreak 2009/2010 IAFP 2011, 7th     European     Symposium on Food Safety, Ede, Netherlands, 18-20, May 2011. p 11.

Fuchs, S., Mester, P., Wagner, M., Rossmanith, P.
The influence of Sample Preparation Chemistry on Bacterial Target Cells Analyzed by     Transmission Electron Microscopy of Listeria monocytogenes and Salmonella     Thyphimurium. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p100.

Roeder, B., Wagner, M., Rossmanith, P.
Application of a novel single bacterial cell manipulation technique to Listeria. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p101.

Roeder, B., Wagner, M., Rossmanith, P.
Stability of calibration function (standards) in nucleic acid based food-pathogen     detection. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p103.

Rossmanith, P., Fuchs, S., Mester, P., Wagner, M.
The fluorescence characteristics of enrichment media in the wavelength range of real-    time PCR thermo-cycler optical pathways. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p100.

Rossmanith, P. and Wagner, M.
Statistical data analysis of real-time PCR results derived from single copy amplification. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p128.

Mester, P., Wagner, M., Rossmanith, P.
Application of ionic liquids for separation and concentration of food-borne pathogens from     food for subsequent molecular or cultural quantification methods. 2nd ÖGMBT Annual     Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p97.

Mester, P., Wagner, M., Rossmanith, P.
Biased spectroscopic protein quantification in the presence of ionic liquids. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria2010. SEP 27-29, Vienna, AUSTRIA, p97.

Rossmanith, P., Frühwirth, K., Fuchs, S., Mester, P., Wagner, M.
Cloning and characterization of a Δ-prfA Listeria monocytogenes strain containing a single copy genomic artificial internal amplification control (IAC) for use as internal sample process control. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p99.
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Rossmanith, P., Frühwirth, K., Süß, B., Schopf, E., Wagner, M.
Micrococcus roseus and Serratia marcescens as coloured bacterial indicators: A simple strategy during design and development of a new method for sample pre-treatment. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p99.

Rossmanith, P., Wagner, M.
Aspects of systems theory in the analysis of molecular-biological based detection     methods. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p103.

Schoder, D., Roeder, B., Wagner, M., Rossmanith, P.
Performance testing of selective enrichment media for L. monocytogenes using single     bacterial cell manipulation. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p102.

Rossmanith, P; Mester, P; Wagner, M; Schoder, D
Demonstration of the effective performance of a combined enrichment/real-time PCR     method targeting the prfA gene of Listeria monocytogenes by testing fresh naturally     contaminated acid curd cheese. 2nd ÖGMBT Annual Meeting 2010; Molecular and     Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA, p98.

Rossmanith, P. and Wagner, M.
Christian Doppler Laboratory for Molecularbiological Food Analytics: Vorstellung des     Christian Doppler Labors. 2nd ÖGMBT Annual Meeting 2010; Molecular and Applied Biosciences Austria 2010. SEP 27-29, Vienna, AUSTRIA.

Schoder, D., Roeder, B., Wagner, M., Rossmanith, P.
Performance testing of selective enrichment media for L. monocytogenes using single bacterial cell manipulation. IAFP 2010, 6th European Symposium on Food Safety, Dublin, Ireland, 9.6.-11.6.2010, p 56.

Rossmanith, P., Frühwirth, K., Fuchs, S., Mester, P., Wagner, M.
Cloning and characterization of a Δ-prfA Listeria monocytogenes strain containing a single copy genomic artificial internal amplification control (IAC) for use as internal sample process control. IAFP 2010, 97th Annual Meeting Anaheim, California, 1.8.-4.8.2010, JFPRDR 73 (Sup) p 87.

Schoder, D., Roeder, B., Wagner, M., Rossmanith, P.
Performance testing of selective enrichment media for L. monocytogenes using single bacterial cell manipulation. IAFP 2010, 97th Annual Meeting Anaheim, California, 1.8 - 4.8.2010, JFPRDR 73 (Sup) p 93.

Roeder, B., Wagner, M., Rossmanith, P.
Stability of calibration function (standards) in nucleic acid based food-pathogen     detection. IAFP 2010, 97th Annual Meeting Anaheim, California, 1.8 - 4.8.2010, JFPRDR 73 (Sup) p 94.

Rossmanith, P., Frühwirth, K., Süß, B., Schopf, E., Wagner, M.
Micrococcus roseus and Serratia marcescens as coloured bacterial indicators: A simple strategy during design and development of a new method for sample pre-treatment. IAFP 2010, 97th Annual Meeting Anaheim, California, 1.8-4.8.2010, JFPRDR 73 (Sup) p 94.

Rossmanith, P. and Wagner, M.
Statistical data analysis of real-time PCR results derived from single copy amplification. IAFP 2010, 97th Annual Meeting Anaheim, California, 1.8-4.8.2010, JFPRDR 73 (Sup) p 94.

Fuchs, S., Mester, P., Wagner, M., Rossmanith, P.
The influence of Sample Preparation Chemistry on Bacterial Target Cells Analyzed by     Transmission Electron Microscopy of Listeria monocytogenes and Salmonella     Thyphimurium. IAFP 2010, 97th Annual Meeting Anaheim, California, 1.8-4.8.2010, JFPRDR 73 (Sup) p 125.

Rossmanith, P., Frühwirth, K., Fuchs, S., Mester, P., Wagner, M.
Cloning and characterization of a Δ-prfA Listeria monocytogenes strain containing a single copy genomic artificial internal amplification control (IAC) for use as internal sample process control. IAFP 2010, 6th European Symposium on Food Safety, Dublin, Ireland, 9.6.-11.6.2010, p 55.

Rossmanith, P., Wagner, M.
Aspects of systems theory in the analysis of molecular-biological based detection     methods. IAFP 2010, 6th European Symposium on Food Safety, Dublin, Ireland, 9.6.-    11.6.2010, p 54.

Schoder, D., Roeder, B., Wagner, M., Rossmanith, P.
Performance testing of selective enrichment media for L. monocytogenes using single bacterial cell manipulation. IAFP 2010, 6th European Symposium on Food Safety, Dublin, Ireland, 9.6.-11.6.2010, p 56.

Mayrl E., Mester P., Wagner, M., Rossmanith, P.
Die Lebend/Tot – Problematik beim Nachweis bakterieller Zellen mittels molekularer     Diagnostik: Implikation und Auswirkungen auf die Entwicklung von Matrix-Lysis als     Probenvorbereitungsmethode
31. Jahrestagung der Österr. Gesellschaft für Hygiene, Mikrobiologie und Präventivmedizin, Bad Ischl, 26.-29.05.2008, S. 79-80.

Roeder, B., Wagner, M., Rossmanith, P.
Application of a novel single bacterial cell manipulation technique to Listeria. FEMS, 3rd Congress of European Microbiologists, Gothenborg, 28.6.-3.7. 2009, p 1988.

Rossmanith, P., Fuchs, S., Mester, P., Wagner, M.
The fluorescence characteristics of enrichment media in the wavelength range of real-    time PCR thermo-cycler optical pathways, FEMS, 3rd Congress of European     Microbiologists, Gothenborg, 28.6.-3.7. 2009, p 1971.
   
Mester, P., Röder, B., Hühn, S., Wagner, M., Rossmanith, P.
Matrix lysis as a sample pre-treatment method facilitating direct quantification of food-    borne pathogens from food and clinical samples with subsequent molecular or cultural quantification methods FEMS, 3rd Congress of European Microbiologists, Gothenborg, 28.6.-3.7. 2009, p 1997.

Hühn, S., Bunge, C., Guerra, B., Wagner, M., Rossmanith, P., Helmuth, R., Malorny, B.
DNA Microarray for characterization and typing of salmonella enterica subsp. Enterica: a novel tool for risk analysís FEMS, 3rd Congress of European Microbiologists, Gothenborg, 28.6.-3.7. 2009, p 1935.

Roeder, B., Wagner, M., Rossmanith, P.
Application of a novel single bacterial cell manipulation technique to Listeria. IAFP 2009, 96th Annual Meeting Grapvine,Texas, 12.7.-15.7.2009, p 184.

Hühn, S., Bunge, C., Guerra, B., Wagner, M., Rossmanith, P., Helmuth, R., Malorny, B.
DNA Microarray for characterization and typing of salmonella enterica subsp. Enterica: a novel tool for risk analysis. IAFP 2009, 96th Annual Meeting Grapvine,Texas, 12.7.-15.7.2009, p 143.

Roeder, B., Wagner, M., Rossmanith, P.
Application of a novel single bacterial cell manipulation technique to Listeria. IAFP 2009, 5th European Symposium on Food Safety, Berlin, Germany, 7.9.-9.9.2009, p 18.

Mester, P., Röder, B., Mayrl, E., Hühn, S., Wagner, M., Rossmanith, P.
Matrix lysis as a sample pre-treatment method facilitating direct quantification of food-    borne pathogens from food and clinical samples with subsequent molecular or cultural quantification methods.  IAFP 2009, 5th European Symposium on Food Safety, Berlin, Germany, 7.9.-9.9.2009, p 20.

Roeder, B., Wagner, M., Rossmanith, P.
Stability of calibration function (standards) in nucleic acid based food-pathogen     detection.. IAFP     2009, 5th European Symposium on Food Safety, Berlin, Germany, 7.9.-9.9.2009, p 18.

Rossmanith, P. and Wagner, M.
Statistical data analysis of real-time PCR results derived from single copy amplification. IAFP 2009, 5th European Symposium on Food Safety, Berlin, Germany, 7.9.-9.9.2009, p 19.

Rossmanith, P., Frühwirth, K., Süß, B., Schopf, E., Wagner, M.
Micrococcus roseus and Serratia marcescens as coloured bacterial indicators: A simple strategy during design and development of a new method for sample pre-treatment. IAFP 2009, 5th European Symposium on Food Safety, Berlin, Germany, 7.9.-9.9.2009, p 19.

PUBLICATIONS (SUPERVISED THESES)

Mayrl, E.
(2009): Matrixlyse als neue Probenvorbereitungsstrategie zum Nachweis pathogener     Mikroorganismen aus Lebensmittel. Dissertation, Vet. Med. Univ. Wien, pp. 54.

Röder, B.
(2009): Improvement of the integration of molecular pathogene testing in the         analytical chain. Dissertation. Vet. Med. Univ. Wien, pp. 121.

Mester, P.
(2008): Anwendung von ionischen Flüssigkeiten im Probenvorbereitungssystem     Matrix Lysis. Diploma Thesis. University Bremen. pp. XX.

AWARDS

Overall winner: Poster award, IAFP 2009, 5th European Symposium on Food Safety, Berlin, Germany, 7.9.-9.9.2009: Rossmanith, P., Frühwirth, K., Süß, B., Schopf, E., Wagner, M. Micrococcus roseus and Serratia marcescens as coloured bacterial indicators: A simple strategy during design and development of a new method for sample pre-treatment.

„Hygienepreis 2007“ of the „Austrian Society for Hygiene, Microbiology and Preventive Medicine“ („Österreichischen Gesellschaft für Hygiene, Mikrobiologie und Präventivmedizin“, ÖGHMP) funded by Unilever.

PATENTS

  • Rossmanith, P., Fuchs, S.,Mester, P., Wagner, M. 2011. “Method for lysing cells” EPA11003449.3
  • European. Patent Application: Nr. EPA10005731.4 “A genetically modified bacterium of the species Listeria monocytogenes”
  • Internat. Patent application: Nr. PCT/AT2007/000388 “Method for isolating cells (Matrix Lysis)”, Earlier Application: A 1346/2006 (AT)
  • European. Patent application: Nr. EPA09163492.3 “Device for singularizing Micro-Objects” 
  • European. Patent application: Nr. EPA09007959.1 “Method for isolating viable cells”
  • European. Patent application: Nr. EPA09010584.2 “Method for isolating viruses”

PUBLICATIONS (others)

  • „Wie kommt das Bakterium aus dem Käse“ News; Nr. 40/2008, S 79.
  • Nominee Wiener Zukunftspreis Profil; Nr. 41/2008, S 125.
  • Broschüre der AWS (Austria Wirtschaftsservice) gemeinsam mit BMWFI und BMWF. Uni:invent - Die Erfolgsstory. Die Erfolgsgeschichte von der Umsetzung universitärer Forschung in die wirtschaftliche Praxis 2004-2009.
    Beitrag Vetmeduni: CD-MOFA, Sichere Lebensmittel – Molekulare Lebensmittelanalytik: ein erfolgreicher Brückenschlag zwischen Grundlagenforschung und industrieller Anwendung.

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