LSM 510 Meta – confocal laser scanning microscope
System Description
The LSM 510 Meta is a confocal laser scanning microscope from Zeiss on an inverted Axiovert 200M stand. All components are controlled by the ZEN 2009 software. It is able to run multidimensional experiments including multichannel acquisition, z-stacks, and time-lapse. Since it is an inverted microscope, it is suitable for imaging both live cells and fixed samples. It is equipped with eight laser lines and four laser detectors for detecting both fluorescence signals and transmitted light DIC.
Widefield Illumination
Halogen lamp HAL 100
Mercury lamp HBO 100
Laser Lines
Laser Diode 405
Argon 458, 477, 488, 514
HeNe 543
HeNe 633
Objectives
5x/0.16 Plan-Apochromat
20x/0.75 Plan-Apochromat
25x/0.8 LCI Plan-Neofluar, DIC
40x/1.30 EC Plan-Neofluar, Oil, DIC
63x/1.40 Plan-Apochromat, Oil, DIC
Filters
Ex 365/12 Em LP 397
Ex 450-490 Em LP 515
Ex 546/12 Em LP 590
Laser Detectors
ChS (META) 411-754
Ch2 420-570
Ch3 505-615
ChD T-PMT (transmitted light)