Observer Z1 – inverted widefield microscope

 
 
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System Description

The Observer Z1 is an inverted microscope from Zeiss allowing brightfield, phase, DIC, and fluorescence microscopy. It is also equipped for live cell imaging experiments. Except the stage the system is fully motorized, and all components are controlled by the ZEN 2012 (blue) software. It is able to run multidimensional experiments including multichannel acquisition, z-stacks, and time-lapse. Since it is an inverted microscope, it is suitable for imaging both live cells and fixed samples. It is equipped with a highly sensitive black&white fluorescence camera.

 

Widefield Illumination

Halogen lamp HAL 100
Illuminator HXP 120 C

Objectives

4x/0.10 Achroplan
10x/0.25 A-Plan, Ph1
20x/0.75 Plan-Apochromat
40x/0.60 LD Plan-Neufluar, Ph2
40x/1.3 Plan-Neofluar, Oil, DIC III
63x/1.4 Plan-Apochromat, Oil, DIC III

Filters

#49   Ex 365                  Em 445/50
#10   Ex 450-490           Em 515-565
#43   Ex 550/25             Em 605/70
#50   Ex 640/30             Em 690/50

Camera

Hamamatsu Orca Flash 4.0

Live Cell Imaging

Humidifier
Digital controller for CO2 concentration, air flow temperature, and chamber heating
Analog controller for objective heating