We investigate cytoskeletal dynamics during equine wound healing, focusing on mature actin stress fibres in equine dermal myofibroblasts. Utilizing complementary in-vitro lesion models, we combine imaging techniques (advanced microscopy) and western blotting to map the spatial organisation and time-dependent dynamics of stress fibres following mechanical disruption. By addressing these structural mechanisms, we strive to add puzzle pieces to the field of equine tissue repair, especially in the case of aberrant wound healing.

Building on our expertise in skin explants, we develop advanced three-dimensional bovine skin equivalents. We believe in the need for robust in vitro platforms to investigate host-pathogen interactions and tissue responses without the need for live animal testing. By bridging the gap between conventional cell culture and in vivo complexity, our work directly contributes to the 3R principles while advancing diagnostic and therapeutic solutions for farm animal health. We here seek to dissect the early cellular events of bovine skin infections such as digital dermatitis under controlled parameters.