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Tissue homogenisation:
Optimised amounts of ceramic, steel or glass beads prefilled in 2 ml screw-cap tubes are used to homogenise different tissue types on the MagNALyzer (Roche Diagnostics)

 

Semiautomated sample processing:
Low throughput sample processing can be performed on the sample processing platform QIAcube (Qiagen). This platform allows to fully automate the processing of almost all Qiagen consumable products. The platform can be used with more than 100 spin column–based protocols for DNA, RNA, and protein sample processing.

 

2100 Bioanalyzer:
The 2100 bioanalyzer (Agilent Technologies) is the most frequently used microfluidics-based platform available, offering solutions for the analysis of DNA, RNA, proteins and cells. Within 30 minutes high quality digital data are delivered. For example, the quality/integrity of RNA can be assessed based on its RNA Integrity Number (RIN)

 

PCR/Melting Curve Analysis:
Q
ualitative sequence detection is used for example to identify pathogens or to determine the sex of higher mammals etc

Publication:
Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci, BMC Microbiology

Digital PCR:
Digital technology has made its way into PCR, allowing researchers to perform absolute quantification of a target sequence by simply counting the number of reactions that contain a positive signal.

Publication:
Cellular and Site-Specific Mitochondrial Characterization of Vital Human Amniotic Membrane, Cell Transplant.

High-resolution amplicon melting:Known and unknown SNPs can be identified by HRM analysis on the Rotor-Gene 6000 (Corbett Life Science) or the LightCycler® 480 System (Roche)

 

Quantification of SNP sequence variants:
ARMS- or REMS-qPCR allow quantification of SNP allelic frequencies down to 0.1 and 0.02%, respectively.

Publication:
Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer, BMC Developemental Biology

Assay design:
Design of various real-time PCR formats for detection of DNA, mRNA or small RNAs like miRNA etc. Either probe-based formats (molecular beacons, TaqMan probes, TaqMan probes incorporating Locked Nucleic Acid (LNA) monomers, TaqMan minor groove binder probes) or fluorogenic double-stranded DNA-binding dye-based formats can be offered.

 

Automated liquid handling:
Profiling of nucleic acid sequences can be cost-efficiently performed on the 384-well microtiter plate format using the epMotion® 5075 LH (Eppendorf) for automated liquid handling and the high-accuracy/sensitivity of qPCR for qualitative or quantitative detection. epMotion-Song (Video)

 

Low density RNA profiling:
Up to 384 mRNA or miRNA can be quantified on custom TaqMan Low Density Arrays (TLDAs, Applied Biosystems)

 

Single-cell analysis:
Analysis of nucleic acid sequences in a single cell can be performed in a microtiter plate format following cell sorting by flow cytometry. 

Meta-analysis is used for summarising RNA expression data e.g. multiple microarray studies
Publication: Cross-Platform Microarray Meta-Analysis for the Mouse Jejunum Selects Novel Reference Genes with Highly Uniform Levels of Expression

Next-Generation sequencing:
Analysis by next-generation sequencing (NGS), for example based on sequencing by synthesis technology of Illumina, is outsourced to sequencing facility partners.

 

UV light is a major DNA-damage agent causing frame shifts and reducing the cloning efficiency. Blue light LEDs developed for the use of non ethidium bromide dyes are as sensitive as UV light but they won't damage the DNA separated in an agarose gel and protect your skin and eyes. A comparison of ​the biologically safer dyes​ Midori Green and ​GelGreenTM is presented here.