Proteomics

Die Proteomics CoreFacility ist eine zentrale Forschungseinrichtung für Protein-Massenspektrometrie. Kontaktieren Sie uns, wenn Sie die Proteinzusammensetzung Ihrer Proben herausfinden wollen. Neben der Proteinidentifizierung, können wir auch Gruppen von Proben quantitativ vergleichen, um Unterschiede im Proteome zu zeigen. Wir helfen gerne, die passende Analysemethode für Ihre spezifische Forschungsfrage zu finden, sei es die Analyse komplexer Zelllysate oder die Proteinanalyse aus 1- or 2-dimensionalen SDS-PAGE Gelen.

For the analysis a high-resolution mass spectrometer (Orbitrap Q Exactive HF)
coupled to a nano-HPLC system (Ultimate 3000 RSLC) from Thermo is available.

Our service includes:

Help and advice during project planning and application for grants
Sample preparation for mass spectrometric analysis: Digest of extracted proteins with various digestion protocols
Analysis of peptides using nano-HPLC coupled to our high-resolution mass spectrometer (Orbitrap Q Exactive HF)
Database search in available protein databases (from public servers as NCBI or UniProt) as well as your own protein database in fasta format
A detailed report of your results containing all the information you need for publication
Help and advice during manuscript writing
We can also provide a basic statistical analysis (t-test) if required. For any further statistical analysis, please contact a biostatistician.

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