The Nikon Eclipse Ti2-E is a spinning disk confocal microscope for live cell imaging and applications requiring high-speed multidimensional imaging.

Objektives:
CFI Plan Apo. λ 10X/ 0.45/ 4.00
CFI Plan Apo. λ 20X/ 0.75/ 1.00
CFI Plan Apo. λ 40X/ 0.95/ 0.25-0.16
CFI Plan Apo. λ Oil 60X/ 1.40/ 0.13
CFI P-Apochromat VC 60x WI /1.20/0.31-0.28

CFI Plan Apo. 10XC Glyc/ 0.50/ 2.00
CFI Plan Apo. λ S 25XC Sil/ 1.05/ 0.55
CFI Plan Apo. λ S 40XC Sil/ 1.25/ 0.30

CFI Apo. LWD λ S 20XC WI/ 0.95/ 0.95

Illumination:
TI2-D-LHLED High-power LED
Lumencor SPECTRA III light engine

Fluorescence Filter:
Nikon DS-Qi2:
LED-DA/FI/TR/Cy5/Cy7-5X-A Filter Cube 32
LED-CFP/YFP/mCherry-3X-A Filter Cube 32

Photometrics Prime BSI:
447/60 BrightLine single-band BP (DAPI)
525/50 BrightLine single-band BP (FITC)
600/52 BrightLine single-band BP (TRITC)
708/75 BrightLine single-band BP (Cy5)

Laser Lines:
405/488/561/638nm (120/100/100/100mW)

Cameras:
Photometrics Prime BSI (two cameras)
Nikon DS-Qi2

Spinning Disk Unit:
Yokogawa CSU-W1
Double disk (25 µm, 50 µm pinhole)
Motorized widefield bypass

Piezo z-drive:
Mad City Labs Nanopositioner
Range of motion: 500 µm
Resolution: 1.0 nm

Live Cell Imaging:
Okolab Cage Incubator
TI2-N-WID Water Immersion Dispenser

The microscope is accessible to authorized persons only. Instrument training is provided upon request and is provided by core facility personnel.

General conditions of equipment usage:

• For operation, it is mandatory to book the Nikon Spinning Disk Ti2-E microscope in the booking calendar.
• The instrument can be operated only by a single user at a time.
• After the experiment, all slides must be removed from the instrument. If used, oil objectives have to be cleaned with lens paper.
• Users must remove their data from the data storage within 3 months after the imaging experiment.

Lab rules:

• Access to the microscopy lab is possible with a keycard, which is granted after completed training on one of the microscopes.
• General S1 lab rules apply.

Further details on laboratory rules and instrument usage will be provided in the training sessions.

The software Nikon NIS-Elements Viewer for viewing the images can be downloaded from the manufacturer's homepage.