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University of Veterinary Medicine Vienna

Zeiss LSM 880 Airyscan – confocal laser scanning microscope

The LSM 880 Airyscan is a confocal laser scanning microscope from Zeiss on an inverted Observer Z1 stand. All components are controlled by the ZEN 2 (black) software. It is able to run multidimensional experiments including multichannel acquisition, z-stacks, tile scanning, and time-lapse. Since it is an inverted microscope, it is suitable for imaging both live cells and fixed samples. It is equipped with six laser lines, detectors for detecting both fluorescence signals and transmitted light DIC, and a z-piezo.

Illumination

Halogen lamp HAL 100
Illuminator HXP 120 V

Laser Lines

Laser Diode | 405
Argon | 458, 488, 514
DPSS | 561
HeNe | 633

Laser Detectors

Ch1 (PMT) | 371 - 740
ChS1 (GaAsP) | 409 - 695
Ch2 (PMT) | 379 - 759
T-PMT | Transmitted Light
ChA | Airyscan

Objectives

10x/0.45 Plan-Apochr.
20x/0.8 Plan-Apochromat
40x/1.40 Plan-Apochr., Oil, DIC III
40x/1.2 LD LCI Plan-Apochromat autocorr., Silicone
63x/1.40 Plan-Apochr., Oil, DIC III

Filters

#49 | Ex 365 | Em 445/50
#38 | Ex 470/40 | Em 525/50
#43 | Ex 550/25 | Em 605/70

Airyscan
beam splitter

458
458/514
458/561
488
488/561
488/561/633

Airyscan
emission filter

BP 420-480 | BP 495-550
BP 420-480 | BP 495-620
BP 420-480 | LP 605
BP 465-505 | LP 525
BP 495-550 | LP 570
BP 570-620 | LP 645

Live cell imaging

Incubator XL dark
TempModul S1
CO2-Modul S1

Further information: BMBWF Forschungsinfrastruktur

 

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