Genomics

 
Isolation tubes with beads

Tissue homogenisation:
O
ptimised amounts of ceramic, steel or glass beads prefilled in 2 ml screw-cap tubes are used to homogenise different tissue types on the MagNALyzer (Roche Diagnostics)

 
Qiacube isolation robot

Semiautomated sample processing:
Low throughput sample processing can be performed on the sample processing platform QIAcube (Qiagen). This platform allows to fully automate the processing of almost all Qiagen consumable products. The platform can be used with more than 100 spin column–based protocols for DNA, RNA, and protein sample processing.

 

PluriSelect:
The innovative separation technology developed by pluriSelect allows isolation of cells, proteins, viruses, bacteria or a variety of other targets from whole blood, cell culture, cord blood or other heterogeneous biological fluids. This technology platform i
s based on the physical separation of differently sized particles using a set of innovative tools. E.g., multiple cell types can be isolated from whole blood within 30 min for RNA and protein analysis.

Video: http://www.pluriselect.de/en/index.htm 1

 
Lab on the chip

2100 Bioanalyzer:
The 2100 bioanalyzer (Agilent Technologies) is the most frequently used microfluidics-based platform available, offering solutions for the analysis of DNA, RNA, proteins and cells. Within 30 minutes high quality digital data are delivered. For example, the quality/integrity of RNA can be assessed based on its RNA Integrity Number (RIN)

 
DNA melting curve analysis

PCR/Melting Curve Analysis:
Q
ualitative sequence detection is used for example to identify pathogens or to determine the sex of higher mammals etc.

Publication:
Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci 2, BMC Microbiology

 

Digital PCR:
Digital technology has made its way into PCR, allowing researchers to perform absolute quantification of a target sequence by simply counting the number of reactions that contain a positive signal.

Publication:
Cellular and Site-Specific Mitochondrial Characterization of Vital Human Amniotic Membrane 3, Cell Transplant.

 
High-resolution melting analysis

High-resolution amplicon melting: Known and unknown SNPs can be identified by HRM analysis on the Rotor-Gene 6000 (Corbett Life Science) or the LightCycler® 480 System (Roche)

 
Quantification of SNP sequences

Quantification of SNP sequence variants:
ARMS- or REMS-qPCR allow quantification of SNP allelic frequencies down to 0.1 and 0.02%, respectively.

Publication:
Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer 4, BMC Developemental Biology

 
RNA and DNA assay design (general image)

Assay design:
Design of various real-time PCR formats for detection of DNA, mRNA or small RNAs like miRNA etc. Either probe-based formats (molecular beacons, TaqMan probes, TaqMan probes incorporating Locked Nucleic Acid (LNA) monomers, TaqMan minor groove binder probes) or fluorogenic double-stranded DNA-binding dye-based formats can be offered.

 
EpMotion 5075TMX

Automated liquid handling:
Profiling of nucleic acid sequences can be cost-efficiently performed on the 384-well microtiter plate format using the epMotion® 5075 LH (Eppendorf) for automated liquid handling and the high-accuracy/sensitivity of qPCR for qualitative or quantitative detection. epMotion-Song 5 (Video)

 
TaqMan low densitiy arrays (TLDA)

Low density RNA profiling:
Up to 384 mRNA or miRNA can be quantified on custom TaqMan Low Density Arrays (TLDAs, Applied Biosystems)

 
AmpliGrid slide

Single-cell analysis:
Analysis of nucleic acid sequences in a single cell can be performed in a microtiter plate format following cell sorting by flow cytometry. 

 

Meta-analysis is used for summarising RNA expression data e.g. multiple microarray studies
Publication: Cross-Platform Microarray Meta-Analysis for the Mouse Jejunum Selects Novel Reference Genes with Highly Uniform Levels of Expression 6

 

454 Sequencing:
Sample preparation for ultra-high-throughput DNA sequencing using 454 sequencing technology. This pyrosequencing technology allows to obtain long sequence reads of about 450 bp preferred for species identification. The sequencing run itself is performed by a 454 sequencing facility partner.

 

UV light is a major DNA-damage agent causing frame shifts and reducing the cloning efficiency. Blue light LEDs developed for the use of non ethidium bromide dyes are as sensitive as UV light 7 but they won't damage the DNA separated in an agarose gel and protect your skin and eyes. A comparison of ​the biologically safer dyes​ Midori Green and ​GelGreenTM is presented here 8.

 

Head

Ao. Univ.-Prof. Mag. Dr. Ralf Steinborn
+43 1 25077 3151
+43 664 60257 6382 
e-mail to Ralf Steinborn





 


 

Staff

Martin Hofer

email to Martin Hofer

+43 1 25077 3152


 

News

 9

Der erste Meilenstein in der interdisziplinären Kooperation mit dem Department für Anthropologie der Universität Wien ist erreicht: die Publikation bei der Zeitschrift "Hormones and Behavior" 10 wurde gerade angenommen.


 
 11

Nach getaner Arbeit 
einer abgeschlossenen 
Klinikkooperation 12.

Download pdf 13


 
 14

Nach getaner Arbeit
einer abgeschlossenen 
Klinikkooperation 12


 
 15

Veränderte und virulentere Keuchhusten-Bakterien 16 auch in Österreich